Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine
Identifieur interne : 003028 ( Main/Exploration ); précédent : 003027; suivant : 003029Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine
Auteurs : Noton Kumar Dutta [Corée du Sud] ; Kaushiki Mazumdar [Corée du Sud] ; Byoung-Hee Lee [Corée du Sud] ; Min-Won Baek [Corée du Sud] ; Dong-Jae Kim [Corée du Sud] ; Yi-Rang Na [Corée du Sud] ; Sung-Hoon Park [Corée du Sud] ; Hyun-Kyoung Lee [Corée du Sud] ; Hiroaki Kariwa [Japon] ; Le Quynh Mai [Viêt Nam] ; Jae-Hak Park [Corée du Sud]Source :
- Immunology Letters [ 0165-2478 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Anticorps (immunologie), Escherichia coli (génétique), Escherichia coli (métabolisme), Femelle, Humains, Lignée cellulaire, Lymphocytes T (cytologie), Lymphocytes T (immunologie), Nucléocapside (génétique), Nucléocapside (immunologie), Nucléocapside (métabolisme), Prolifération cellulaire, Souris, Souris de lignée BALB C, Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins antiviraux (métabolisme), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Vaccins à ADN (métabolisme), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (métabolisme), Épitopes (immunologie).
- MESH :
- cytologie : Lymphocytes T.
- génétique : Escherichia coli, Nucléocapside, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS.
- immunologie : Anticorps, Lymphocytes T, Nucléocapside, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS, Épitopes.
- métabolisme : Escherichia coli, Nucléocapside, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS.
- Animaux, Femelle, Humains, Lignée cellulaire, Prolifération cellulaire, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies (immunology), Cell Line, Cell Proliferation, Epitopes (immunology), Escherichia coli (genetics), Escherichia coli (metabolism), Female, Humans, Mice, Mice, Inbred BALB C, Nucleocapsid (genetics), Nucleocapsid (immunology), Nucleocapsid (metabolism), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), T-Lymphocytes (cytology), T-Lymphocytes (immunology), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Vaccines, DNA (metabolism), Viral Vaccines (genetics), Viral Vaccines (immunology), Viral Vaccines (metabolism).
- MESH :
- chemical , genetics : Vaccines, DNA, Viral Vaccines.
- chemical , immunology : Antibodies, Epitopes, Vaccines, DNA, Viral Vaccines.
- cytology : T-Lymphocytes.
- genetics : Escherichia coli, Nucleocapsid, SARS Virus.
- immunology : Nucleocapsid, SARS Virus, T-Lymphocytes.
- metabolism : Escherichia coli, Nucleocapsid, SARS Virus, Vaccines, DNA, Viral Vaccines.
- Animals, Cell Line, Cell Proliferation, Female, Humans, Mice, Mice, Inbred BALB C.
Abstract
It is believed today that nucleocapsid protein (N) of severe acute respiratory syndrome (SARS)-CoV is one of the most promising antigen candidates for vaccine design. In this study, three fragments [N1 (residues: 1–422); N2 (residues: 1–109); N3 (residues: 110–422)] of N protein of SARS-CoV were expressed in
Url:
DOI: 10.1016/j.imlet.2008.03.003
PubMed: 18440652
PubMed Central: 7112843
Affiliations:
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Le document en format XML
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type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName 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gene for the design of an epitope-based SARS DNA vaccine</title><author><name sortKey="Dutta, Noton Kumar" sort="Dutta, Noton Kumar" uniqKey="Dutta N" first="Noton Kumar" last="Dutta">Noton Kumar Dutta</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Mazumdar, Kaushiki" sort="Mazumdar, Kaushiki" uniqKey="Mazumdar K" first="Kaushiki" last="Mazumdar">Kaushiki Mazumdar</name><affiliation wicri:level="4"><nlm:aff id="aff2">Department of Microbiology and Immunology, College of Medicine, Seoul National University, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Microbiology and Immunology, College of Medicine, Seoul National University</wicri:regionArea><placeName><settlement type="city">Séoul</settlement></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name><affiliation wicri:level="4"><nlm:aff id="aff3">Department of Animal Experimentation, College of Medicine, Seoul National University, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Animal Experimentation, College of Medicine, Seoul National University</wicri:regionArea><placeName><settlement type="city">Séoul</settlement></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Baek, Min Won" sort="Baek, Min Won" uniqKey="Baek M" first="Min-Won" last="Baek">Min-Won Baek</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author><author><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name><affiliation wicri:level="1"><nlm:aff id="aff4">Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo</wicri:regionArea><wicri:noRegion>Sapporo</wicri:noRegion></affiliation></author><author><name sortKey="Mai, Le Quynh" sort="Mai, Le Quynh" uniqKey="Mai L" first="Le Quynh" last="Mai">Le Quynh Mai</name><affiliation wicri:level="1"><nlm:aff id="aff5">Virology Department, National Institute of Hygiene and Epidemiology, Yersin Street, Hanoi, Viet Nam</nlm:aff><country xml:lang="fr">Viêt Nam</country><wicri:regionArea>Virology Department, National Institute of Hygiene and Epidemiology, Yersin Street, Hanoi</wicri:regionArea><wicri:noRegion>Hanoi</wicri:noRegion></affiliation></author><author><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name><affiliation wicri:level="4"><nlm:aff id="aff1">Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, San 56-1, Sillim 9 Dong, Gwanak-gu, Seoul 151742</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName><placeName><settlement type="city">Séoul</settlement></placeName></affiliation></author></analytic><series><title level="j">Immunology Letters</title><idno type="ISSN">0165-2478</idno><idno type="eISSN">1879-0542</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies (immunology)</term><term>Cell Line</term><term>Cell Proliferation</term><term>Epitopes (immunology)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid (genetics)</term><term>Nucleocapsid (immunology)</term><term>Nucleocapsid (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (metabolism)</term><term>T-Lymphocytes (cytology)</term><term>T-Lymphocytes (immunology)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term><term>Vaccines, DNA (metabolism)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term><term>Viral Vaccines (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps (immunologie)</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Lymphocytes T (cytologie)</term><term>Lymphocytes T (immunologie)</term><term>Nucléocapside (génétique)</term><term>Nucléocapside (immunologie)</term><term>Nucléocapside (métabolisme)</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins antiviraux (métabolisme)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Vaccins à ADN (métabolisme)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (métabolisme)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies</term><term>Epitopes</term><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Lymphocytes T</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Nucleocapsid</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Nucléocapside</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps</term><term>Lymphocytes T</term><term>Nucléocapside</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term><term>Virus du SRAS</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Nucleocapsid</term><term>SARS Virus</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Nucleocapsid</term><term>SARS Virus</term><term>Vaccines, DNA</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Nucléocapside</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cell Proliferation</term><term>Female</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>It is believed today that nucleocapsid protein (N) of severe acute respiratory syndrome (SARS)-CoV is one of the most promising antigen candidates for vaccine design. In this study, three fragments [N1 (residues: 1–422); N2 (residues: 1–109); N3 (residues: 110–422)] of N protein of SARS-CoV were expressed in <italic>Escherichia coli</italic> and analyzed by pooled sera of convalescence phase of SARS patients. Three gene fragments [N1 (1–1269 nt), N2 (1–327 nt) and N3 (328–1269 nt)—expressing the same proteins of N1, N2 and N3, respectively] of SARS-N were cloned into pVAX-1 and used to immunize BALB/c mice by electroporation. Humoral (by enzyme-linked immunosorbent assay, ELISA) and cellular (by cell proliferation and CD4<sup>+</sup>:CD8<sup>+</sup> assay) immunity was detected by using recombinant N1 and N3 specific antigen. Results showed that N1 and N3 fragments of N protein expressed by <italic>E. coli</italic> were able to react with sera of SARS patients but N2 could not. Specific humoral and cellular immunity in mice could be induced significantly by inoculating SARS-CoV N1 and N3 DNA vaccine. In addition, the immune response levels in N3 were significantly higher for antibody responses (IgG and IgG1 but not IgG2a) and cell proliferation but not in CD4<sup>+</sup>:CD8<sup>+</sup> assay compared to N1 vaccine. The identification of antigenic N protein fragments has implications to provide basic information for the design of DNA vaccine against SARS-CoV. The present results not only suggest that DNA immunization with pVax-N3 could be used as potential DNA vaccination approaches to induce antibody in BALB/c mice, but also illustrates that gene immunization with these SARS DNA vaccines can generate different immune responses.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. 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Azlinda</name></author><author><name sortKey="Kun, Y" uniqKey="Kun Y">Y. Kun</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li><li>Japon</li><li>Viêt Nam</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Dutta, Noton Kumar" sort="Dutta, Noton Kumar" uniqKey="Dutta N" first="Noton Kumar" last="Dutta">Noton Kumar Dutta</name></region><name sortKey="Baek, Min Won" sort="Baek, Min Won" uniqKey="Baek M" first="Min-Won" last="Baek">Min-Won Baek</name><name sortKey="Kim, Dong Jae" sort="Kim, Dong Jae" uniqKey="Kim D" first="Dong-Jae" last="Kim">Dong-Jae Kim</name><name sortKey="Lee, Byoung Hee" sort="Lee, Byoung Hee" uniqKey="Lee B" first="Byoung-Hee" last="Lee">Byoung-Hee Lee</name><name sortKey="Lee, Hyun Kyoung" sort="Lee, Hyun Kyoung" uniqKey="Lee H" first="Hyun-Kyoung" last="Lee">Hyun-Kyoung Lee</name><name sortKey="Mazumdar, Kaushiki" sort="Mazumdar, Kaushiki" uniqKey="Mazumdar K" first="Kaushiki" last="Mazumdar">Kaushiki Mazumdar</name><name sortKey="Na, Yi Rang" sort="Na, Yi Rang" uniqKey="Na Y" first="Yi-Rang" last="Na">Yi-Rang Na</name><name sortKey="Park, Jae Hak" sort="Park, Jae Hak" uniqKey="Park J" first="Jae-Hak" last="Park">Jae-Hak Park</name><name sortKey="Park, Sung Hoon" sort="Park, Sung Hoon" uniqKey="Park S" first="Sung-Hoon" last="Park">Sung-Hoon Park</name></country><country name="Japon"><noRegion><name sortKey="Kariwa, Hiroaki" sort="Kariwa, Hiroaki" uniqKey="Kariwa H" first="Hiroaki" last="Kariwa">Hiroaki Kariwa</name></noRegion></country><country name="Viêt Nam"><noRegion><name sortKey="Mai, Le Quynh" sort="Mai, Le Quynh" uniqKey="Mai L" first="Le Quynh" last="Mai">Le Quynh Mai</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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